Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase
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منابع مشابه
Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase eta.
The Saccharomyces cerevisiae RAD30 gene functions in error-free replication of UV-damaged DNA. RAD30 encodes a DNA polymerase, Pol eta, which inserts two adenines opposite the two thymines of a cis-syn thymine-thymine (T-T) dimer. Here we use steady-state kinetics to determine the accuracy of DNA synthesis opposite the T-T dimer. Surprisingly, the accuracy of DNA synthesis opposite the damaged ...
متن کاملMechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase eta.
DNA polymerase eta (Poleta) has the unique ability to replicate through UV-light-induced cyclobutane pyrimidine dimers. Here we use pre-steady-state kinetic analyses to examine the mechanism of nucleotide incorporation opposite a cis-syn thymine-thymine (TT) dimer and an identical nondamaged sequence by yeast Poleta. Poleta displayed "burst" kinetics for nucleotide incorporation opposite both t...
متن کاملDistinct mechanisms of cis-syn thymine dimer bypass by Dpo4 and DNA polymerase eta.
UV-light-induced cyclobutane pyrimidine dimers (CPDs) present a severe block to synthesis by replicative DNA polymerases (Pols), whereas Poleta promotes proficient and error-free replication through CPDs. Although the archael Dpo4, which, like Poleta, belongs to the Y family of DNA Pols, can also replicate through a CPD, it is much less efficient than Poleta. The x-ray crystal structure of Dpo4...
متن کاملDirect observation of thymine dimer repair in DNA by photolyase.
Photolyase uses light energy to split UV-induced cyclobutane dimers in damaged DNA, but its molecular mechanism has never been directly revealed. Here, we report the direct mapping of catalytic processes through femtosecond synchronization of the enzymatic dynamics with the repair function. We observed direct electron transfer from the excited flavin cofactor to the dimer in 170 ps and back ele...
متن کاملThymine Dimers - Dna Lesions Induced by Sunlight Cis-syn Thymine Dimer Phosphoramidite Now Available
3900 COLUMNS One of the major sources of DNA damage in all organisms is the UV component of sunlight. The predominant reaction induced by UV light on DNA is dimerization of adjacent pyrimidine bases leading to cyclobutane dimers (CPDs) and 6-4 photoproducts. The dimers formed in the most significant quantity are the cis-syn cyclobutane dimer of two thymine bases (1) and the corresponding 64 pho...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2000
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.97.7.3094